The Effect of White Pigmentation in 96 Well Plates during QPCR

QPCR instruments are able to monitor amplicon quantity in real-time during PCR reactions by detecting fluorescence signals and recording fluorescence data. The majority of fluorescence is reflected out of the QPCR plate either by the polypropylene itself or by the walls of the thermal cycler block, when natural polypropylene is used. QPCR plates containing a white pigment improve reflection towards the fluorescence detector inside the thermocycler during a QPCR run (Figure 1 and Figure 2).