Sartorius Introduces Revolutionary Microscope

The NucleoCounter NC-3000 is an automated full-spectrum fluorescence microscope that offers a wide array of reliable and user-friendly assays with integrated collection, analysis and reporting of data.

Validated for mammalian, yeast, insect and fish cells, the NucleoCounter NC-3000 is designed for applications including viability and cell count, cell cycle assay, mitochondrial potential assay, vitality assay, quantification of fluorescence intensity, fluorescence measurements from violet to far red, DNA fragmentation assay and GFP transfection efficiency assay. It will be of interest to those working in areas such as stem cell research, cell counting and viability, apoptosis and cancer, vaccine production, gene expression as well as transfection and cell cycle studies.

The system is easy to set up with no special training needed; users simply prepare the sample, load it into the NC‐3000 and choose the required assay. It is also extremely fast with an analysis time of 15‐60 seconds (1μl sample) – this enables a complete viability assay to be performed within two minutes – including sample handling. Unlike flow cytometers there are no fluids within the NC‐3000 which means no clogging, no spillage and no maintenance. 

 
Benefits of the NucleoCounter NC-3000 include:

·            Excellent precision and reproducibility

·            Extreme ease of use

·            Fast analysis (very short total operation time)

·            Automated data analysis and superior data visualisation software

·            Calibration, maintenance and service free

·            All-in-One instrument with a small footprint

·            Plug & Play System – preset parameters

                 

Key Specifications of the NucleoCounter NC-3000:

Optics
    Lens with x2 magnification, 1/2” CCD with 1360 x 1024 pixels

Sample device
    NC-3000 cassettes
    NC-3000 disposable chamber slides (2, 4 and 8 chambers per slide)

Excitation (nm)
    Seven LED light sources with peaks at 365, 405, 455, 475, 500, 525 and 590 (standard version)

Emission (nm)
    Eight interchangeable emission filters: 410-450, 415-525, 450-500, 500-555,520-595, 555-605, 595-750, 630-1100 (standard version)

Sample consumption
    50μl (cassettes)
< 30μl (chamber slides)

Analysed volume
    1-4μl sample (cassettes)
    0.2-20μl sample (chamber slides)

Optimal Range
    5 x 104 - 1 x 107 cells/ml

Cell types
    Mammalian cells, yeast, insect cells, fish cells

Released applications
    Cell count and viability
    Transfection efficiency (GFP)
    Cell cycle analysis
    Apoptosis Assays

PC Platform

     Microsoft Windows 32 bit XP SP3, USB 2.0, Screen minimum 1024 x 768, recommended 2GB RAM

Analysis time
    Typical 15-60 seconds (1μl sample)

Data presentation
    Images, tables, histograms, scatter plots

Weight
    14 kg

Dimensions
    29 x 29 x 31 cm (H x W x D)

Supply Voltage
    100-240V~ 50-60Hz

Power Consumption
    5/50W (power save mode/peak)

 

Availability

The Chemometec NucleoCounter NC-3000 is immediately available from Sartorius.